Central buds as well as lateral buds from the corms of field grown cultivars of Gajendra and Bidhan Kusum are cultured on Murashige and Skoog (MS) medium in the presence of various combinations of growth supplements. Callus found after 2 weeks. Pink opaque compact callus are maintained in dark condition for 12 weeks and when it turns into light green, it is transferred to the light for shoot initiation. Multiple shoot formation and shoot elongation is found in presence of glucose (2mg/l) and L-ascorbic acid (20mg/l). Plantlets are successfully hardened in potting mixture containing soil, cocopeat, vermicompost in 1:1:1 ratio. Plantlets survival rate was observed 100% in hardening condition.